Development of a transposon mutagenesis system in the oral spirochete Treponema denticola.
نویسندگان
چکیده
Here, we report successful transposon mutagenesis in the oral spirochete Treponema denticola. A modified Himar1 transposon, including a new antibiotic selection cassette for T. denticola, generated mutations affecting cell division, transport, and chemotaxis, among other processes. This random mutagenesis system should facilitate research on the biology and pathogenesis of this spirochete, which is associated with human periodontal diseases.
منابع مشابه
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 74 20 شماره
صفحات -
تاریخ انتشار 2008